| dc.description.abstract | Introduction and aim: The aim of our research was to examine the effect of tramadol on presence
of stress-induced ulcer lesions, as well as pathohistological alterations of gastric mucosa in rats
exposed to cold restraint stress (CRS).
Material and methods: The experiment was performed on male Wistar rats, weighing 220g,
exposed to cold restraint stress (CRS). Animals was pretreated with tramadol (Tramadol®) in the
doses of 100mg/kg body weight, injected intraperitoneally, just before cold restraint stress. The
total size of lesions was expressed as ulcer index (UI). The pathohistological samples were
analyzed by Leica DML S2 light microscope.
Results: In animals exposed to CRS there was macroscopically observed deep, dark erosions,
and the UI was 4,98±6,18mm 2 . The presence of shallow and deep defects of the mucosa was
pathohistological confirmed, but also the presence of extravasated erythrocytes and hyperemic
blood vessels. In the submucosa and l.m. mucosae the signs of inflammation were noted, i.e.,
edema and mass of leucocytes (polymorphonuclears). Tramadol decreased overall surface of the
alterations in statistically significant manner, i.e., UL from 4,98 mm 2 to 0,02±0,04 mm 2
(p<0,001). The mucosal defects of the mucosa were observed pathohistological, on the surface
itself, but without accompanying bleeding. In the submucosal and l-m. mucosae hyperemia, as
well as mass of leucocytes and mastocytes were noted. On the surface of the mucosa, painted
with PAS painted mucosa cells and abundant presence of mucosa.
Conclusion: Pre-treatment with tramadol can prevent the development of ulcer lesions, and thus
if influences gastric pathohistological alterations in stressed rats. | en_US |
